Journal: Histopathology

Article Title: A new algorithm for coeliac disease based on the 'long forgotten' TCRγδ + intra-epithelial lymphocytes detected with an antibody working on FFPE sections.

doi: 10.1111/his.15330

Figure Lengend Snippet: Figure 1. IELs determined by haematoxylin and eosin (H&E), CD3 and T cell receptor (TCR)δ stains in all study groups. Sample cases for each study group [from top to bottom: controls, non-CD intra-epithelial lymphocytosis (IELosis), type 1, type 2, type 3 CD] are presented in rows and stains (from left to right; H&E, CD3, TCR δ) are presented in columns.

Article Snippet: I M M U N O H I S T O C H E M I C A L E V A L U A T I O N Four μm-thick consecutive sections were stained using rabbit polyclonal anti-CD3 antibody (Cell Marque, Rocklin, CA, USA, dilution: 1:100) and TCR δ antibody (clone H-41; Santa Cruz, Dallas, TX, USA, dilution: 1:200) recognising both major chains, δ1 and δ2 on FFPE material with streptavidin biotin– peroxidase to determine the number of T cells and γδ+ cells, respectively, in the surface/villus epithelial compartment.

Techniques:

Journal: Histopathology

Article Title: A new algorithm for coeliac disease based on the 'long forgotten' TCRγδ + intra-epithelial lymphocytes detected with an antibody working on FFPE sections.

doi: 10.1111/his.15330

Figure Lengend Snippet: Figure 3. A 63-year-old patient with positive occult blood in the stool, for whom serological and genetic information was unavailable, under- went endoscopy due to clinical suspicion of CD. Edema and erythema were observed in the duodenal mucosa. In H&E stained sections, 25 IELs (a), 29 IELs in CD3 stained sections (b), and 4 IELs in TCR stained sections (c) per 100 enterocytes were identified. When the discrimi- nant formula was applied, the highest value was observed in the control (normal) group. The application of the formula is presented below.

Article Snippet: I M M U N O H I S T O C H E M I C A L E V A L U A T I O N Four μm-thick consecutive sections were stained using rabbit polyclonal anti-CD3 antibody (Cell Marque, Rocklin, CA, USA, dilution: 1:100) and TCR δ antibody (clone H-41; Santa Cruz, Dallas, TX, USA, dilution: 1:200) recognising both major chains, δ1 and δ2 on FFPE material with streptavidin biotin– peroxidase to determine the number of T cells and γδ+ cells, respectively, in the surface/villus epithelial compartment.

Techniques: Staining, Control

Journal: Histopathology

Article Title: A new algorithm for coeliac disease based on the 'long forgotten' TCRγδ + intra-epithelial lymphocytes detected with an antibody working on FFPE sections.

doi: 10.1111/his.15330

Figure Lengend Snippet: Figure 1. IELs determined by haematoxylin and eosin (H&E), CD3 and T cell receptor (TCR)δ stains in all study groups. Sample cases for each study group [from top to bottom: controls, non-CD intra-epithelial lymphocytosis (IELosis), type 1, type 2, type 3 CD] are presented in rows and stains (from left to right; H&E, CD3, TCR δ) are presented in columns.

Article Snippet: I M M U N O H I S T O C H E M I C A L E V A L U A T I O N Four μm-thick consecutive sections were stained using rabbit polyclonal anti-CD3 antibody (Cell Marque, Rocklin, CA, USA, dilution: 1:100) and TCR δ antibody (clone H-41; Santa Cruz, Dallas, TX, USA, dilution: 1:200) recognising both major chains, δ1 and δ2 on FFPE material with streptavidin biotin– peroxidase to determine the number of T cells and γδ+ cells, respectively, in the surface/villus epithelial compartment.

Techniques:

Journal: Histopathology

Article Title: A new algorithm for coeliac disease based on the 'long forgotten' TCRγδ + intra-epithelial lymphocytes detected with an antibody working on FFPE sections.

doi: 10.1111/his.15330

Figure Lengend Snippet: Figure 3. A 63-year-old patient with positive occult blood in the stool, for whom serological and genetic information was unavailable, under- went endoscopy due to clinical suspicion of CD. Edema and erythema were observed in the duodenal mucosa. In H&E stained sections, 25 IELs (a), 29 IELs in CD3 stained sections (b), and 4 IELs in TCR stained sections (c) per 100 enterocytes were identified. When the discrimi- nant formula was applied, the highest value was observed in the control (normal) group. The application of the formula is presented below.

Article Snippet: I M M U N O H I S T O C H E M I C A L E V A L U A T I O N Four μm-thick consecutive sections were stained using rabbit polyclonal anti-CD3 antibody (Cell Marque, Rocklin, CA, USA, dilution: 1:100) and TCR δ antibody (clone H-41; Santa Cruz, Dallas, TX, USA, dilution: 1:200) recognising both major chains, δ1 and δ2 on FFPE material with streptavidin biotin– peroxidase to determine the number of T cells and γδ+ cells, respectively, in the surface/villus epithelial compartment.

Techniques: Staining, Control